Ciita^tm1.1Dim
Targeted Allele Detail

Summary

• Symbol: Ciita^tm1.1Dim
• Name: class II transactivator; targeted mutation 1.1, Diane Mathis
• MGI ID: MGI:3664552
• Synonyms: CIITA⁰
• Gene: Ciita Location: Chr16:10297923-10346282 bp, + strand Genetic Position: Chr16, 5.65 cM, cytoband B1
• Alliance: Ciita^tm1.1Dim page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:51885
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A single loxP site was introduced 5' to exon 2 and a loxP-flanked thymidine kinase-neomycin fusion gene was inserted 3' to exon 3. Cre-mediated deletion of the intervening sequences was accomplished by mating mutant mice to CMV-cre mice, deleting the DNA encoding the major transactivation domain. RT-PCR analysis on spleen and thymus RNA indicated that no detectable transcript is expressed from this allele. (J:51885)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Ciita^tm1.1Dim/Ciita^tm1.1Dim	involves: 129S2/SvPas * C57BL/6 * SJL

Phenotypes:

Affected Systems	hm1
show or hide all annotated terms
hematopoietic system	√
decreased dendritic cell number	√
decreased CD4-positive, alpha-beta T cell number	√
immune system	√
decreased dendritic cell number	√
decreased CD4-positive, alpha-beta T cell number	√
abnormal level of surface class II molecules	√

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ciita Mutation: 46 strains or lines available

References

• Original: J:51885 Williams GS, et al., Mice lacking the transcription factor CIITA--a second look. Int Immunol. 1998 Dec;10(12):1957-67
• All: 1 reference(s)