Tg(Gfap-TK)7.1Mvs
Transgene Detail

Summary

• Symbol: Tg(Gfap-TK)7.1Mvs
• Name: transgene insertion 7.1, Michael V Sofroniew
• MGI ID: MGI:3664567
• Synonyms: Gfap-HSV-Tk, GFAP-TK
• Transgene: Tg(Gfap-TK)7.1Mvs Location: unknown
• Alliance: Tg(Gfap-TK)7.1Mvs page

Transgene origin

• Strain of Origin: (C57BL/10 x CBA)F1 X CFLP

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The Herpes simplex virus thymidine kinase gene, including the polyadenylation signal, was ligated into the first exon of a mouse glial fibrillary acidic protein promoter cassette that contains all the exons, introns and promoter regulatory elements and includes 2.5 kb of 5' and 2 kb of 3' flanking genomic DNA. The GFAP protein is not expressed from the transgene because a small segment of exon one has been deleted. RT-PCR analysis showed co-expression of HSV-Tk and of Gfap mRNA (from the endogenous gene) in heart, lung, liver, spleen, adrenal, kidney and GI tract, as well as in brain and trigeminal ganglion; in neural tissues the co-expressing cells have the appearance of astroglia. (J:104241)

Expression

In Structures Affected by this Mutation:

14 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

Notes

Line 7.1, estimated to carry >50 copies of the transgene, was selected from three original lines (the others unnamed) for further analysis because mice of this line exhibited the greatest HSV thymidine kinase expression in the brain.

Ganciclovir is metabolized to toxic nucleotide analogs in cells expressing HSV-TK. Subcutaneous or intraperitoneal administration of ganciclovir (GCV) or elaidic acid ganciclovir (eGCV) to transgenic mice results in specific ablation of proliferating cells that express GFAP.

References

• Original: J:104241 Bush TG, et al., Fulminant jejuno-ileitis following ablation of enteric glia in adult transgenic mice. Cell. 1998 Apr 17;93(2):189-201
• All: 26 reference(s)