Unc5a^tm1Lhck
Targeted Allele Detail

Summary

• Symbol: Unc5a^tm1Lhck
• Name: unc-5 netrin receptor A; targeted mutation 1, Lindsay Hinck
• MGI ID: MGI:3665134
• Gene: Unc5a Location: Chr13:55097224-55153831 bp, + strand Genetic Position: Chr13, 29.8 cM
• Alliance: Unc5a^tm1Lhck page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:111725
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 4-8 were replaced with a cassette containing IRES-TauLacZ and neomycin phosphotransferase (Neo) sequences. RT-PCR analysis on spinal cord RNA derived from homozygous mice showed that the encoded transcript was not present. Immunohistochemistry on spinal cord sections from homozygous mice demonstrated a loss of immunoreactivity. (J:111725)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Unc5a Mutation: 32 strains or lines available

References

• Original: J:111725 Williams ME, et al., UNC5A promotes neuronal apoptosis during spinal cord development independent of netrin-1. Nat Neurosci. 2006 Aug;9(8):996-8
• All: 6 reference(s)