Acta2^tm1Rjs
Targeted Allele Detail

Summary

• Symbol: Acta2^tm1Rjs
• Name: actin alpha 2, smooth muscle, aorta; targeted mutation 1, Robert J Schwartz
• MGI ID: MGI:3665248
• Gene: Acta2 Location: Chr19:34218490-34232990 bp, - strand Genetic Position: Chr19, 29.41 cM, cytoband C3
• Alliance: Acta2^tm1Rjs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:113261
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A pol2neobpA cassette was inserted into the locus between the promoter and the coding region to disrupt transcription. Northern blots confirmed absence of transcript in mutant aorta, intestine and uterus. Western blot confirm absence of protein in mutant aorta, intestine and uterus. (J:113261)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Acta2 Mutation: 57 strains or lines available

References

• Original: J:113261 Schildmeyer LA, et al., Impaired vascular contractility and blood pressure homeostasis in the smooth muscle alpha-actin null mouse. FASEB J. 2000 Nov;14(14):2213-20
• All: 11 reference(s)