Ifng^tm1Lky
Targeted Allele Detail

Summary

• Symbol: Ifng^tm1Lky
• Name: interferon gamma; targeted mutation 1, Richard M Locksley
• MGI ID: MGI:3665254
• Synonyms: Yeti
• Gene: Ifng Location: Chr10:118276951-118281797 bp, + strand Genetic Position: Chr10, 66.75 cM
• Alliance: Ifng^tm1Lky page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:113265
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S4/SvJae-Tg(Prm-cre)70Og

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: An IRES element, eYFP and a bovine polyA sequence followed by a floxed neomycin resistance cassette were inserted into the end of the coding sequence. Targeting took place in ES cells homozygous for Tg(Prm-cre)70Og, which express Cre recombinase in male germ cells. Cre-mediated excision subsequently removed the neomycin cassette upon transmission from male chimeric mice. The protamine-cre transgene was separated from the knock-in allele by crossing germline male mutant mice with C57BL/6 or BALB/c mice. (J:113265)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ifng Mutation: 49 strains or lines available

References

• Original: J:113265 Stetson DB, et al., Constitutive cytokine mRNAs mark natural killer (NK) and NK T cells poised for rapid effector function. J Exp Med. 2003 Oct 6;198(7):1069-76
• All: 32 reference(s)