Apob^tm1Mae
Targeted Allele Detail

Summary

• Symbol: Apob^tm1Mae
• Name: apolipoprotein B; targeted mutation 1, Nobuyo Maeda
• MGI ID: MGI:3665449
• Synonyms: apo b81
• Gene: Apob Location: Chr12:8027648-8066835 bp, + strand Genetic Position: Chr12, 3.53 cM
• Alliance: Apob^tm1Mae page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:36426
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: Using an 'in step' recombination strategy, the sequence encoding the putative binding domains in exon 26 were replaced with those encoding human HBB peptides. The 5' binding domain (3143LSVKAQYKKNSD3154) was replaced with amino acids 1-12 of the HBBS globin (1VHLTPVEKSAVT12). The 3' binding domain (3356GTSRLMRKRGL3366) was replaced with amino acids 120-131 of HBBA globin (120KEFTPPVQAAYQ131). The reading frame was maintained in the mutant allele, however, the disruption creates a premature stop codon at position 3688. The mutant allele contains 3687 residues, which is 81% of the full length protein. (J:36426)

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Apob Mutation: 224 strains or lines available

References

• Original: J:36426 Toth LR, et al., Two distinct apolipoprotein B alleles in mice generated by a single 'in-out' targeting. Gene. 1996 31;178(1-2):161-8
• All: 3 reference(s)