Pax3^tm5Buck
Targeted Allele Detail

Summary

• Symbol: Pax3^tm5Buck
• Name: paired box 3; targeted mutation 5, Margaret E Buckingham
• MGI ID: MGI:3687384
• Synonyms: Pax3^ERD, Pax3^{GFP(Pax3-En-IRESnlacZ)}, Pax3^Pax3-ERD
• Gene: Pax3 Location: Chr1:78077904-78173771 bp, - strand Genetic Position: Chr1, 39.79 cM
• Alliance: Pax3^tm5Buck page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:112275
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Conditional ready, Dominant negative, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: A sequence encoding a floxed GFP-FRT-puro cassette followed by a form of Pax3 that acts as a transcriptional repressor was targeted to the locus. The repressor sequence contains the N-terminus of the Pax3 DNA-binding domains fused to the repression domain of the Drosophila Engrailed transcriptional repressor. Cre mediated recomibination results in the loss of the floxed GFP-FRT-Puro cassette and creates a dominant negative allele. (J:112275)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pax3 Mutation: 50 strains or lines available

References

• Original: J:112275 Bajard L, et al., A novel genetic hierarchy functions during hypaxial myogenesis: Pax3 directly activates Myf5 in muscle progenitor cells in the limb. Genes Dev. 2006 Sep 1;20(17):2450-64
• All: 3 reference(s)