Map2k6^tm1Ina
Targeted Allele Detail

Summary

• Symbol: Map2k6^tm1Ina
• Name: mitogen-activated protein kinase kinase 6; targeted mutation 1, Izumi Nakashima
• MGI ID: MGI:3687978
• Synonyms: MKK6^-
• Gene: Map2k6 Location: Chr11:110289948-110416348 bp, + strand Genetic Position: Chr11, 73.6 cM, cytoband E1
• Alliance: Map2k6^tm1Ina page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:100093
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin resistance cassette was inserted into exon 3, in the region encoding the kinase domain of the protein. RT-PCR analysis detects no transcript from the disrupted gene in thymocyte RNA from homozygous mutant mice. Immunoblot analysis detects no protein product from the targeted gene in thymocyte lysates from homozygous mutant mice and a reduced amount in lysates from heterozygous mutants. (J:100093)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Map2k6 Mutation: 42 strains or lines available

References

• Original: J:100093 Suzuki H, et al., Involvement of MKK6 in TCRalphabeta(int)CD69lo: a target population for apoptotic cell death in thymocytes. FASEB J. 2003 Aug;17(11):1538-40
• All: 1 reference(s)