Mbnl2^Gt(XB595)Byg
Gene trapped Allele Detail

Summary

• Symbol: Mbnl2^Gt(XB595)Byg
• Name: muscleblind like splicing factor 2; gene trap XB595, BayGenomics
• MGI ID: MGI:3688251
• Synonyms: Mbnl2^GT4
• Gene: Mbnl2 Location: Chr14:120513081-120669109 bp, + strand Genetic Position: Chr14, 64.44 cM
• Alliance: Mbnl2^Gt(XB595)Byg page

Mutation origin

• Mutant Cell Line: XB595
• Germline Transmission: Earliest citation of germline transmission: J:112061
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0pfs Vector Type: gene trap
• Mutation details: The pGTOpfs gene trap vector inserted into intron 4 of the locus. This vector contained the En2 splice acceptor site, betageo fused to a neomycin resistance cassette and the SV40 polyA signal. Transcripts from mutants contained the N-terminal and two C3H zinc fingers of Mbnl2 fused in frame to beta-geo. (J:112061)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XB595	CC156314 ; (MGI Seq Detail)	5' RACE	Chr14:120562779-120623000 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  45 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mbnl2 Mutation: 66 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:112061 Lin X, et al., Failure of MBNL1-dependent post-natal splicing transitions in myotonic dystrophy. Hum Mol Genet. 2006 Jul 1;15(13):2087-97
• All: 5 reference(s)