Epha2^{Gt(KST085)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Epha2^{Gt(KST085)Byg}
• Name: Eph receptor A2; gene trap KST085, BayGenomics
• MGI ID: MGI:3688255
• Synonyms: Epha2^-
• Gene: Epha2 Location: Chr4:141028551-141056695 bp, + strand Genetic Position: Chr4, 73.67 cM, cytoband D-E
• Alliance: Epha2^{Gt(KST085)Byg} page

Development of progressive cataract in Epha2^{Gt(KST085)Byg}/Epha2^{Gt(KST085)Byg} mice

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: KST085
• Germline Transmission: Earliest citation of germline transmission: J:112120
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1TMpfs Vector Type: secretory trap
• Mutation details: A gene trap vector was inserted between exons 5 and 6, leading to the truncation of exons 6 to 17 encoding the second fibronectin type III repeat in the extracellular domain all the way to thecarboxyl terminal end. The remaining ectodomain encoded by exons 1 to 5 was fused to neo and betagalactosidase. (J:112120)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  KST085	CC144754 ; (MGI Seq Detail)	5' RACE	Chr4:141044315-141044513 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  78 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Epha2 Mutation: 96 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:112120 Guo H, et al., Disruption of EphA2 receptor tyrosine kinase leads to increased susceptibility to carcinogenesis in mouse skin. Cancer Res. 2006 Jul 15;66(14):7050-8
• All: 4 reference(s)