Tg(Tcrb2B4)1Mmd
Transgene Detail

Summary

• Symbol: Tg(Tcrb2B4)1Mmd
• Name: transgene insertion 1, Mark M Davis
• MGI ID: MGI:3688272
• Synonyms: 2B4betaEH, 2B4beta Tg, Vbeta3
• Transgene: Tg(Tcrb2B4)1Mmd Location: unknown
• Alliance: Tg(Tcrb2B4)1Mmd page

Transgene origin

• Strain of Origin: (C57BL/6J x C3H/HeJ)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Tcrb2B4)1Mmd expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tcrb (MGI:98578)

• Mutation details: The rearranged 2B4 beta chain with 4.5 kb of 5' sequence was cleaved at the EcoRV site in the 3' untranslated exon of Cbeta and a XhoI linker was inserted. The resulting 9.3 kb fragment extending from the 5' end of the clone was ligated to a 3 kb fragment carrying the 3' end of the C beta 2. the immunoglobin heavy-chain enhancer was nserted immediately downstream of the Jbeta2.5 segment. (J:93694)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:93694 Berg LJ, et al., Antigen/MHC-specific T cells are preferentially exported from the thymus in the presence of their MHC ligand. Cell. 1989 Sep 22;58(6):1035-46
• All: 18 reference(s)