Tg(Sp7-tTA,tetO-EGFP/cre)1Amc
Transgene Detail

Summary

• Symbol: Tg(Sp7-tTA,tetO-EGFP/cre)1Amc
• Name: transgene insertion 1, Andrew P McMahon
• MGI ID: MGI:3689350
• Synonyms: osterix-Cre, Osx1-cre, Osx1-Cre, Osx1-GFP::Cre, Osx-Cre, Osx-Cre::GFP, Osx-gfp, The Sp7-Cre-EGFP
• Transgene: Tg(Sp7-tTA,tetO-EGFP/cre)1Amc Location: unknown
• Alliance: Tg(Sp7-tTA,tetO-EGFP/cre)1Amc page

Transgene origin

• Strain of Origin: CD-1

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase, Reporter, Transactivator)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(Sp7-tTA,tetO-EGFP/cre)1Amc expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Sp7 (MGI:2153568)

• Mutation details: A tTA-regulated GFP::Cre construct, which has the tetracycline-regulated transactivator (tTA) cassette upstream of a tetracycline-responsive element (tetO)-controlled Enhanced Green Fluorescent Protein/cre fusion protein was used to modify a bacterial artificial chromosome (BAC) encoding exon 1 of the mouse Sp7 gene (formerly osterix or Osx1). An frt-flanked selection cassette following the tTA-regulated GFP::Cre was removed from correctly targeted bacteria via transient flpe expression. A 5.2 kb portion of the modified BAC (Osx1-GFP::Cre) was used for pronuclear injection into fertilized CD1 embryos. (J:114494)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Sp7 (mouse) Summary of all recombinase alleles driven by Sp7. Comparative matrix view of recombinase activities Alliance mouse Sp7 gene page

Disease models

Expression

In Mice Carrying this Mutation:	20 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Tg(Sp7-tTA,tetO-EGFP/cre)1Amc

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

References

• Original: J:114494 Rodda SJ, et al., Distinct roles for Hedgehog and canonical Wnt signaling in specification, differentiation and maintenance of osteoblast progenitors. Development. 2006 Aug;133(16):3231-44
• All: 302 reference(s)