Chat^tm1(cre)Lowl
Targeted Allele Detail

Summary

• Symbol: Chat^tm1(cre)Lowl
• Name: choline O-acetyltransferase; targeted mutation 1, Bradford B Lowell
• MGI ID: MGI:3689420
• Synonyms: ChAT-IRES-Cre
• Gene: Chat Location: Chr14:32130160-32187866 bp, - strand Genetic Position: Chr14, 19.4 cM
• Alliance: Chat^tm1(cre)Lowl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:114556
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Chat^tm1(cre)Lowl expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Chat (MGI:88392)

• Mutation details: An optimized internal ribosome entry sequence (IRES) fused to cre recombinase followed by a neomycin resistance cassette flanked FRT sites was inserted after the ChAT stop codon in BAC (bMQ185k21) using homologous recombination in bacteria. A genomic ChAT fragment with 4kb homology arms on either side of the IRES-Cre insertion site was lifted out from the BAC using reverse recombineering techniques and transformed into ES cells. The neo cassette was excised with Flp recombinase. Cre recombinase activity is reported in all cholinergic neurons. This allele generated from Chat^tm2(cre)Lowl. (J:114556, J:169562)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Chat (mouse) Summary of all recombinase alleles driven by Chat. Comparative matrix view of recombinase activities Alliance mouse Chat gene page

Expression

In Mice Carrying this Mutation:

33 assay results 2 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Chat Mutation: 58 strains or lines available

References

• Original: J:114556 Lowell BB, et al., Development and phenotype of ChAT-IRES-Cre mice. MGI Direct Data Submission. 2006
• All: 140 reference(s)