Tg(tetO-EGFR*L858R)57Hev
Transgene Detail

Summary

• Symbol: Tg(tetO-EGFR*L858R)57Hev
• Name: transgene insertion 57, Harold E Varmus
• MGI ID: MGI:3690075
• Synonyms: EGFR^L858R, L858R57, TetO-EGFR^L858R
• Transgene: Tg(tetO-EGFR*L858R)57Hev Location: unknown
• Alliance: Tg(tetO-EGFR*L858R)57Hev page

Transgene origin

• Strain of Origin: (C57BL/6 x CBA)F2

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: A mutant human epidermal growth factor receptor (EGFR) cDNA with a T-to-G nucleotide substitution in exon 21 that replaces leucine with arginine at amino acid position 858 (L858R) was cloned between a tetracycline-responsive element and an intron and polyadenylation signal from the mouse protamine 1 gene. Mice with this transgene that express the tetracycline transactivator (tTA) or reverse transactivator (rtTA) protein under the control of a tissue-specific promoter can be induced to express the mutant EGFR in tissues or cell types of interest by administration/withdrawal of doxycycline. Transgene expression in this line is correlated with a head-to-tail array of transgenes and not with other, independently segregating transgene copies. (J:109092)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Tg(tetO-EGFR*L858R)57Hev

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Line 56 was also generated.

References

• Original: J:109092 Politi K, et al., Lung adenocarcinomas induced in mice by mutant EGF receptors found in human lung cancers respond to a tyrosine kinase inhibitor or to down-regulation of the receptors. Genes Dev. 2006 Jun 1;20(11):1496-510
• All: 3 reference(s)