Pax3^tm5.1Buck
Targeted Allele Detail

Summary

• Symbol: Pax3^tm5.1Buck
• Name: paired box 3; targeted mutation 5.1, Margaret E Buckingham
• MGI ID: MGI:3690080
• Synonyms: Pax3^Pax3-En, Pax3^{PAX3-En-IRESnLacZ}
• Gene: Pax3 Location: Chr1:78077904-78173771 bp, - strand Genetic Position: Chr1, 39.79 cM
• Alliance: Pax3^tm5.1Buck page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:112275
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Dominant negative, Reporter)
• Mutation: Insertion
• Mutation details: Cre mediated recombination removed the floxed GFP-FRT-puro cassette from Pax3^tm5Buck allowing expresion of a form of Pax3 that acts as a transcriptional repressor. The repressor sequence contains the N-terminus of the Pax3 DNA-binding domains fused to the repression domain of the Drosophila Engrailed transcriptional repressor. LacZ expression is seen in Pax3 expressing cells. (J:112275)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pax3 Mutation: 50 strains or lines available

Notes

This allele was generated in J:112275 using a cross between females carrying Tg(Pgk1-cre)1Lni and males carrying the floxed allele. As this cre transgene is expressed in diploid eggs, all offspring are exposed to Cre protien, independent of inheritance of the cre transgene.

References

• Original: J:112275 Bajard L, et al., A novel genetic hierarchy functions during hypaxial myogenesis: Pax3 directly activates Myf5 in muscle progenitor cells in the limb. Genes Dev. 2006 Sep 1;20(17):2450-64
• All: 9 reference(s)