Cd151^tm1Nki
Targeted Allele Detail

Summary

• Symbol: Cd151^tm1Nki
• Name: CD151 antigen; targeted mutation 1, The Netherlands Cancer Institute
• MGI ID: MGI:3691076
• Synonyms: Cd151^-, Cd151^tm1Son
• Gene: Cd151 Location: Chr7:141047305-141051386 bp, + strand Genetic Position: Chr7, 86.82 cM
• Alliance: Cd151^tm1Nki page

Glomerular basement membrane abnormalities and foot process effacement in kidneys of mildly affected Cd151^tm1Nki/Cd151^tm1Nki and Itga3^tm1Son/Itga3^tm1Son Tg(NPHS2-cre)295Lbh/0 mice

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:114988
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 2. An FRT-flanked puromycin resistance cassette with a 3' loxP site was inserted downstream of exon 4. Exons 2-4 were removed via cre-mediated recombination. Immunoblot failed to detect protein in mutant MEFs. The final allele lacks the puromycin resistance cassette. The floxed allele Cd151^tm2Nki was used to generate this allele. (J:114988)

Expression

In Structures Affected by this Mutation:

10 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd151 Mutation: 34 strains or lines available

References

• Original: J:114988 Sachs N, et al., Kidney failure in mice lacking the tetraspanin CD151. J Cell Biol. 2006 Oct 9;175(1):33-9
• All: 3 reference(s)