Scn5a^{tm1(SCN5A)Rdn}
Targeted Allele Detail

Summary

• Symbol: Scn5a^{tm1(SCN5A)Rdn}
• Name: sodium channel, voltage-gated, type V, alpha; targeted mutation 1, Dan M Roden
• MGI ID: MGI:3691483
• Synonyms: H
• Gene: Scn5a Location: Chr9:119312474-119408082 bp, - strand Genetic Position: Chr9, 71.33 cM
• Alliance: Scn5a^{tm1(SCN5A)Rdn} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:115269
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, RMCE-ready)
• Mutation: Insertion
• Scn5a^{tm1(SCN5A)Rdn} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  SCN5A (6331)

  Scn5a

  (MGI:98251)

• Mutation details: Recombinase-mediated cassette exchange was used to replace exon 2 with a loxP site, the full length human SCN5A, an FRT-flanked hygro cassette and a loxP site. Restriction fragment length polmorphisms were used to confirm that only the human transcripts were expressed in mutant hearts. This knock-in contains the conserved noncoding sequence 28 (CNS28). (J:115269, J:185686)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Scn5a Mutation: 105 strains or lines available

References

• Original: J:115269 Liu K, et al., Recombinase-mediated cassette exchange to rapidly and efficiently generate mice with human cardiac sodium channels. Genesis. 2006 Nov;44(11):556-64
• All: 5 reference(s)