Dgkd^tm1Mtop
Targeted Allele Detail

Summary

• Symbol: Dgkd^tm1Mtop
• Name: diacylglycerol kinase, delta; targeted mutation 1, Matthew K Topham
• MGI ID: MGI:3692387
• Synonyms: Dgkd^-
• Gene: Dgkd Location: Chr1:87781009-87872902 bp, + strand Genetic Position: Chr1, 44.44 cM
• Alliance: Dgkd^tm1Mtop page

Morphology of Dgkd^tm1Mtop/Dgkd^tm1Mtop mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:115297
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 6-8 were replaced with a neomycin resistance gene. These exons encode the second zinc finger motif and part of the catalytic domain. Immunoblot and RT-PCR confirmed absence of expression in mutants. (J:115297)

Expression

In Mice Carrying this Mutation:

20 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dgkd Mutation: 56 strains or lines available

References

• Original: J:115297 Crotty T, et al., Diacylglycerol kinase delta regulates protein kinase C and epidermal growth factor receptor signaling. Proc Natl Acad Sci U S A. 2006 Oct 17;103(42):15485-90
• All: 3 reference(s)