Ctrq1^C3H/HeJ
QTL Variant Detail

Summary

• QTL variant: Ctrq1^C3H/HeJ
• Name: C. trachomatis resistance QTL 1; C3H/HeJ
• MGI ID: MGI:3692648
• QTL: Ctrq1 Location: unknown Genetic Position: Chr2, cM position of peak correlated region/allele: 60.69 cM
• QTL Note: genome coordinates based on the marker associated with the peak LOD score

Variant origin

• Strain of Specimen: C3H/HeJ

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers susceptibility to Chlamydia trachomatis compared to C57BL/6J. (J:106111)
• Inheritance: Recessive

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:106111

Linkage analysis was performed on 169 animals from a (C57BL/6J x C3H/HeJ)F2 intercross to identify loci associated with susceptibility to Chlamydia trachomatis infection. Genome scan was conducted using 93 microsatellite markers at an average spacing of 16 cM. Parental strain C57BL/6J is more resistant to infection with C. trachomatis compared to parental strain C3H/HeJ. Animals were phenotyped by analyzing bacterial load in spleens after infection with C. trachomatis. In vitro analysis was also performed using INF-gamma-treated mouse embryonic fibroblasts (MEFs) derived from (C57BL/6J x C3H/HeJ)F2 animals infected with C. trachomatis.

Ctrq1 (C. trachomatis resistance QTL 1) mapped to 69 cM on mouse Chromosome 2 near D2Mit277 (LRS=20.5). C3H/HeJ-derivedalleles at Ctrq1 confer susceptibility to C. trachomatis infection with a recessive mode of inheritance.

Ctrq2 (C. trachomatis resistance QTL 2) mapped to 55 cM on mouse Chromosome 3 near D3Mit57 (LRS=19.9). The 1.5 LOD support interval spans 44.8 cM to66.2 cM from D3Mit49 to D3Mit350. C3H/HeJ-derived alleles at Ctrq2 confer resistance to C. trachomatis infection with dominant inheritance.

Ctrq3 (C. trachomatis resistance QTL 3) mapped to 32 cM on mouse Chromosome 11 near D11Mit143 (LRS=19.5). C3H/HeJ-derived alleles at Ctrq3 confer susceptibility to C. trachomatis infection with dominant inheritance. This locus was reaffirmed by constructing a congenic line carrying the entire Ctrq3 region (D11Mit20 to D11Mit245) derived from susceptible strain C3H/HeJ on the genetic background of resistant strain C57BL/6J. B6.C3H-Ctrq3 congenics exhibit significantly greater bacterial load after C. trachomatis infection compared to C57BL/6J controls. IFN-gamma-treated MEFs derived from heterozygous B6.C3H-Ctrq3 animals infected with C. trachomatis also exhibit a greater level of bacterial replication compared to controls.

References

• Original: J:106111 Bernstein-Hanley I, et al., Genetic analysis of susceptibility to Chlamydia trachomatis in mouse. Genes Immun. 2006 Mar;7(2):1-8
• All: 1 reference(s)