Ccni^tm1Jro
Targeted Allele Detail

Summary

• Symbol: Ccni^tm1Jro
• Name: cyclin I; targeted mutation 1, James M Roberts
• MGI ID: MGI:3693371
• Synonyms: I^-
• Gene: Ccni Location: Chr5:93329796-93354348 bp, - strand Genetic Position: Chr5, 47.29 cM, cytoband E3.3-F1.3
• Alliance: Ccni^tm1Jro page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:115356
• Parent Cell Line: AK7 (ES Cell)
• Strain of Origin: 129S4/SvJaeSor

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The region of the gene encompassing all the coding exons except a small portion of the last exon was replaced by bacterial neomycin resistance and lacZ genes. No protein product from the targeted gene is detected by immunoblot analysis in tissue extracts of homozygous mutant 14- or 18-days post coitum (dpc) embryos or in mouse embryonic fibroblasts (MEFs) cultured from homozygous 13 dpc embryos. The highest levels of beta-galactosidase expression are detected by immunohistochemical analysis in brain, followed by testis, of one-month-old pups. In the kidney, distinct staining for beta-galactosidase is seen in a podocyte distribution in the glomerulus, and weaker, variable beta-galactosidase expression is seen in cells of the tubules. (J:115356)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ccni Mutation: 24 strains or lines available

References

• Original: J:115356 Griffin SV, et al., Cyclin I protects podocytes from apoptosis. J Biol Chem. 2006 Sep 22;281(38):28048-57
• All: 2 reference(s)