Polq^tm2Jow
Targeted Allele Detail

Summary

• Symbol: Polq^tm2Jow
• Name: polymerase (DNA directed), theta; targeted mutation 2, Jiyang O-Wang
• MGI ID: MGI:3693648
• Gene: Polq Location: Chr16:36832148-36915779 bp, + strand Genetic Position: Chr16, 26.32 cM, cytoband B4
• Alliance: Polq^tm2Jow page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:115989
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Disruption caused by insertion of vector
• Mutation details: Exons 1 through 3, containing the 5' UTR and translation initiation site, were replaced with a neomycin resistance cassette. Northern blot failed to detect transcript in mutants. (J:115989)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Polq Mutation: 127 strains or lines available

References

• Original: J:115989 Masuda K, et al., Absence of DNA polymerase theta results in decreased somatic hypermutation frequency and altered mutation patterns in Ig genes. DNA Repair (Amst). 2006 Nov 8;5(11):1384-91
• All: 2 reference(s)