Tg(Thy1-EGFP)JJrs
Transgene Detail

Summary

• Symbol: Tg(Thy1-EGFP)JJrs
• Name: transgene insertion J, Joshua R Sanes
• MGI ID: MGI:3693894
• Synonyms: GFP-J, Tg(Thy1-GFPJ)1Jrs, Tg(Thy1-GFP)JJrs
• Transgene: Tg(Thy1-EGFP)JJrs Location: unknown
• Alliance: Tg(Thy1-EGFP)JJrs page

Transgene origin

• Strain of Origin: (C57BL/6J x CBA)F1

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: The transgene contains a GFP gene under the direction of regulatory elements derived from the mouse Thy1 gene. Regulatory elements are composed of a 6.5 kb fragment obtained from the 5' portion of the Thy1 gene, extending from the promoter to the intron following exon 4. Exon 3 and its flanking introns are absent. (J:93699)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Transgenic mice express spectral variants of GFP at high levels in motor and sensory neurons, as well as in subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. No expression is detectable in nonneural cells. Fluorescence is stable to mild aldehyde fixation. Expression is strong from a mid-gestational stage into adulthood.

References

• Original: J:93699 Feng G, et al., Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron. 2000 Oct;28(1):41-51
• All: 5 reference(s)