Tg(GNAT2-Dta)98Wwk
Transgene Detail

Summary

• Symbol: Tg(GNAT2-Dta)98Wwk
• Name: transgene insertion 98, Winston W-Y Kao
• MGI ID: MGI:3693959
• Synonyms: h-GNAT2pro-DTA, Tg98, Trc-Tox176
• Transgene: Tg(GNAT2-Dta)98Wwk Location: unknown
• Alliance: Tg(GNAT2-Dta)98Wwk page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The 2.4-kb transgenic construct comprises a 277-base pair (-bp) DNA fragment containing the cone photoreceptor cell-specific promoter from the human GNAT2 gene upstream of a 680-bp cDNA encoding an attenuated diphtheria toxin A-chain followed by an SV40 intron and polyadenylation signal and a 214-bp enhancer element from the human RBP3 gene. While RT-PCR detects transcripts from the endogenous Gnat1 and Gnat2 genes, (encoding the rod and cone transducin alpha subunits, respectively) in wild-type retinas from postnatal day 8 (P8) onward, neither endogenous Gnat2 transcripts nor transgene-derived mRNA are detected in retinas from mice of transgenic line 98 at P8 or later. (J:116305)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Of four transgenic lines derived from independent germline-transmitting transgenic founder mice, only the line descended from male founder C98, estimated by Southern blot analysis to carry approximately 10 tandemly-arrayed copies of the transgene, exhibited a retinal phenotype. Lines derived from female founders C8, C12 and C13, each estimated to carry two tandemly integrated copies of the transgene, exhibited no retinal phenotype even at 180 days of age and so were not characterized further.

References

• Original: J:116305 Ying S, et al., Retinal degeneration in cone photoreceptor cell-ablated transgenic mice. Mol Vis. 2000 Jun 24;6:101-8
• All: 2 reference(s)