Actre6^LP/J
QTL Variant Detail

Summary

• QTL variant: Actre6^LP/J
• Name: activity response to ethanol 6; LP/J
• MGI ID: MGI:3694240
• QTL: Actre6 Location: unknown Genetic Position: Chr9, cM position of peak correlated region/allele: 25.91 cM
• QTL Note: genome coordinates based on the marker associated with the peak LOD score

Variant origin

• Strain of Specimen: LP/J

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers decreased ethanol-induced locomotor activation compared to C57BL/6J. (J:116204)
• Inheritance: Not Specified

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:116204

Multiple cross mapping (MCM) was used to identify QTLs associated with ethanol induced activation of locomotor activity. The following F2 crosses were generated and screened for polymorphic markers: (C57BL/6J x DBA/2J)F2, (DBA/2J x LP/J)F2, (BALB/cJ x LP/J)F2, (BALB/cJ x DBA/2J)F2, (C57BL/6J x BALB/cJ)F2, and (C57BL/6J x LP/J)F2. Animals were administered 1.5 g/kg ethanol and monitored for activity for 20 minutes. Parental strains DBA/2J and BALB/cJ exhibit increased locomotor activity after ethanol challenge whereas parental strains C57BL/6J and LP/J exhibit decreased locomotor activity.

QTL Analysis in the F2 Intercrosses:

Previously identified QTL Actre1 (chr1) was duplicated in this study. This locus is associated with the 2.5-5 minute interval in the (DBA/2J x C57BL/6J)F2 cross with LOD=7.3.

Actre1 was also detected in the (LP/J x C57BL/6)F2 cross with LOD=4.6.

12.09.2015 Curator Note: Because Actre1 was originally mapped in J:70181 in 2001 using both a BXD RI population and an (C57BL/6J x DBA/2J) F2 intercross population, which differs from the (LP/J x C57BL/6J)F2 population, we consider the (LP/J x C57BL/6J)F2 cross a separate mapping experiment and have named the QTL Actre13.

QTL Actre13 maps to mouse Chromosome 1 with a LOD score of 4.3

Another suggestive QTL on Chr 1 was detected in the (BALB/c x C57BL/6J)F2 intercross with LOD=3.4. Homozygosity for C57BL/6J-derived alleles at Actre1, Actre13 and at the suggestive QTL confered increased locomotor activation in all 3 crosses. Analysis of all 3 F2 crosses showed significantgenotype x phenotype interaction at 100 cM near D1Mit150.

Significant linkage to ethanol-induced locomotor activation during the 2.5-5 minute interval mapped to 78.5 cM on mouse Chromosome 3 in the (DBA/2J x BALB/cJ)F2 cross near D3Mit44 (LOD=4.3). Thislocus is named Actre5 (activity response to ethanol 5). BALB/cJ-derived alleles at Actre5 confer increased locomotor activity.

Suggestive linkage ethanol-induced locomotor activity was detected at 58.8 cM in the (LP/J x BALB/cJ)F2 cross near D3Mit216 (LOD=3.3).

Suggestive linkage ethanol-induced locomotor activity was detected at 58.8 cM in the (LP/J x BALB/cJ)F2 cross near D3Mit216 (LOD=3.3).

Actre6 (activity response to ethanol 6) mapped to 27 cM on mouse Chromosome 9 in the (LP/J x C57BL/6J)F2 cross near D9Mit130 (LOD=5.4).This locus is associated with ethanol-induced locomotor activation during the 5-20 minute interval with homozygosity for LP/J-derived alleles conferring decreased locomotor activation.

Suggestive linkage mapped to4 9cM near D9Mit273 (LOD=3) in the (LP/JxBALB/c)F2 cross.

Analysis of heteogeneous

Analysis of heterogeneous stock animals (derived from C57BL/6J, DBA/2J, LP/J, and BALB/cJ) at the G5 generation suggest that 2 QTLs may be present in the Actre1 region at D1Mit355 (97 cM) and at D1Mit218 (67 cM).In both cases the C57BL/6J-derived allele is associated with increased locomotor activity.

Previously identified QTL Actre2 (chr2) was analyzed in this study using heterogenous stock animals (derived from C57BL/6J, DBA/2J, LP/J, and BALB/cJ) at the G5 generation. A maximum LOD score of 5 was attained near D2Mit102-D2Mit62 (52cM - 65 cM) with C57BL/6J-derived alleles conferring increased locomotor activity. SNP analysis of G19 heterogeneous stock animals narrowed the Actre2 interval to a B6-LP:C-D2 biallelic haplotype block between 112.5 Mb and 117.5 Mb.

12.09.2015 Curator Note: Because Actre2 was originally identified in J:71081 in 2001 using a much larger HS sample derived from 8 strains we consider the 4 strain HS stock used here to be a separate mapping experiment mapping a unique QTL labled Actre14.

References

• Original: J:116204 Malmanger B, et al., Further studies on using multiple-cross mapping (MCM) to map quantitative trait loci. Mamm Genome. 2006 Dec;17(12):1193-204
• All: 1 reference(s)