Igkj1^{tm1(IgkHyHEL10)Mnz}
Targeted Allele Detail

Summary

• Symbol: Igkj1^{tm1(IgkHyHEL10)Mnz}
• Name: immunoglobulin kappa joining 1; targeted mutation 1, Michel C Nussenzweig
• MGI ID: MGI:3694788
• Synonyms: Tg(Igk-V22-33-aHEL)1Mnz, VkalphaHEL
• Gene: Igkj1 Location: Chr6:70699546-70699583 bp, + strand Genetic Position: Chr6, 32.04 cM
• Alliance: Igkj1^{tm1(IgkHyHEL10)Mnz} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:115079
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: The recombined immunoglobulin genes present in the HyHEL10 hybridoma produce antibodies with high affinity for hen egg lysozyme (HEL). The recombined murine Igk variable and joining gene segments present in this hybridoma were placed under the control of a Vkappa22-33 promoter. A floxed neomycin resistance cassette was placed upstream and the entire construct replaced the endogenous Igk-J1 gene segment. Mice were crossed with cre-transgenic mice to removed the floxed neomycin cassette. The transgenic IgK light chain is detectable on some B cells in heterozygote mice. (J:115079)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Igkj1 Mutation: 2 strains or lines available

References

• Original: J:115079 Casellas R, et al., Contribution of receptor editing to the antibody repertoire. Science. 2001 Feb 23;291(5508):1541-4
• All: 3 reference(s)