Pon2^Gt(XE661)Byg
Gene trapped Allele Detail

Summary

• Symbol: Pon2^Gt(XE661)Byg
• Name: paraoxonase 2; gene trap XE661, BayGenomics
• MGI ID: MGI:3695400
• Gene: Pon2 Location: Chr6:5264620-5298408 bp, - strand Genetic Position: Chr6, 1.99 cM
• Alliance: Pon2^Gt(XE661)Byg page

Mutation origin

• Mutant Cell Line: XE661
• Germline Transmission: Earliest citation of germline transmission: J:116935
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: The pGT1Lxf gene trap vector, containing a splice-acceptor sequence upstream of a betageo, was inserted into exon 2. The resulting transcript contains exons 1 and 2 from the locus and the betageo. Nortern and Western blot analyses confirmed absence of wild type expression of paraoxonase 2 transcript and protein, respectively. (J:116935)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XE661	CC199571 ; (MGI Seq Detail)	5' RACE	Chr6:5289013-5298340 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  1 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pon2 Mutation: 31 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:116935 Ng CJ, et al., Paraoxonase-2 deficiency aggravates atherosclerosis in mice despite lower apolipoprotein-B-containing lipoproteins: anti-atherogenic role for paraoxonase-2. J Biol Chem. 2006 Oct 6;281(40):29491-500
• All: 17 reference(s)