Napepld^tm1Crv
Targeted Allele Detail

Summary

• Symbol: Napepld^tm1Crv
• Name: N-acyl phosphatidylethanolamine phospholipase D; targeted mutation 1, Benjamin F Cravatt
• MGI ID: MGI:3695401
• Synonyms: NAPE-PLD^-
• Gene: Napepld Location: Chr5:21867899-21906394 bp, - strand Genetic Position: Chr5, 9.97 cM
• Alliance: Napepld^tm1Crv page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:108301
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 4 was replaced with a neomycin resistance gene by homologous recombination. This exon encodes the majority of the protein sequence including the conserved HXHXDH catalytic residues. Absence of transcript and protein was confirmed in RT-PCR and Western blot analyses. (J:108301)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Napepld Mutation: 25 strains or lines available

References

• Original: J:108301 Leung D, et al., Inactivation of N-acyl phosphatidylethanolamine phospholipase D reveals multiple mechanisms for the biosynthesis of endocannabinoids. Biochemistry. 2006 Apr 18;45(15):4720-6
• All: 8 reference(s)