Tg(TetOp-Polb/tTA)2Sbl
Transgene Detail

Summary

• Symbol: Tg(TetOp-Polb/tTA)2Sbl
• Name: transgene insertion 2, Robert W Sobol
• MGI ID: MGI:3695686
• Synonyms: TetOp-Flag_polbeta-tTA
• Transgene: Tg(TetOp-Polb/tTA)2Sbl Location: unknown
• Alliance: Tg(TetOp-Polb/tTA)2Sbl page

Transgene origin

• Strain of Origin: C57BL/6 x SJL

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inducible, Inserted expressed sequence, Transactivator)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: A transgenic construct was made that consisted of the TetOp promoter driving expression of a Flag-tagged beta-polymerase minigene and the tetracycline transactivator (tTA) from a bicistronic mRNA via the EMC virus IRES sequence. Basal levels of expression of the bicistronic mRNA initiate both beta-polymerase and tetracycline transactivator protein expression, thereby generating autocatalytic positive feedback to induce promoter transactivation and strongly increased levels of transgenic protein expression. Analysis of transgene expression (via Flag-epitope immunohistochemistry) in 8-week-old mice demonstrated expression in most tissues, with increased expression in the epithelial cells of the brain, pituitary gland, adrenal glands, oviduct, lens, and skin. (J:117775)

Expression

In Structures Affected by this Mutation:

11 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Tg(TetOp-Polb/tTA)2Sbl

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:117775 Sobol RW, et al., Regulated over-expression of DNA polymerase beta mediates early onset cataract in mice. DNA Repair (Amst). 2003 May 13;2(5):609-22
• All: 2 reference(s)