Krt14^{tm1(Krt10)Pko}
Targeted Allele Detail

Summary

• Symbol: Krt14^{tm1(Krt10)Pko}
• Name: keratin 14; targeted mutation 1, Peter J Koch
• MGI ID: MGI:3696658
• Synonyms: K1014chim
• Gene: Krt14 Location: Chr11:100093988-100098336 bp, - strand Genetic Position: Chr11, 63.43 cM, cytoband D
• Alliance: Krt14^{tm1(Krt10)Pko} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:117347
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: Sequence encoding the N-terminal head domain in exon 1 was replaced with the sequence for the keratin 10 head domain. Exons 7 and 8 were deleted and a cDNA encoding the keratin 10 tail domain was fused with exon 6 of keratin 14. A pgk-neo flanked by loxP sites was introduced into intron 1 and subsequently removed via cre mediated recombination. Expression of keratin 10 was detected by Western blotting, confirming successful creation of the chimeric protein. (J:117347)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Krt14 Mutation: 38 strains or lines available

References

• Original: J:117347 Chen J, et al., An unexpected role for keratin 10 end domains in susceptibility to skin cancer. J Cell Sci. 2006 Dec 15;119(Pt 24):5067-76
• All: 1 reference(s)