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Atf2Tg(Gzma-Klra1)7Wum
Transgenic Allele Detail
Summary
Symbol: Atf2Tg(Gzma-Klra1)7Wum
Name: activating transcription factor 2; transgene insertion 7, Wayne M Yokoyama
MGI ID: MGI:3696689
Synonyms: A-Ly49A transgene, NK-cell-deficient, NKD
Gene: Atf2  Location: Chr2:73646853-73722983 bp, - strand  Genetic Position: Chr2, 43.86 cM
Alliance: Atf2Tg(Gzma-Klra1)7Wum page
Transgene
origin
Strain of Origin:  C57BL/6
Transgene
description
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
 
Atf2Tg(Gzma-Klra1)7Wum expresses 1 gene
 
Mutation detailsA murine granzyme A genomic construct (carrying 11-kb putative promoter and 7-kb structural gene with poly-A addition signal) was used for expression of a Ly49A cDNA. The granzyme A translation start codon was deleted and replaced with a Ly49A cDNA that was inserted into the SmaI site of the modified granzyme A gene. Transgenic mice derived from founder no. 7 expressed Ly49A on nearly all splenic NK1.11 CD32 cells and also expressed Ly49A on approximately one-half of T cells but not on other cell types.

Sequence analysis revealed that the Tg construct inserted into exon 5 of the gene for Atf2. Relative to transcription of Atf 2, the Tg construct is directed in the opposite orientation. Analysis of the 3' integration site indicated a fusion of the Tg construct and intron 10. At this end, the Tg construct is oriented in the same direction as Atf 2. PCR of genomic DNA from Tg mice with primers derived from Atf 2 and the transgenic construct confirmed both integration sites into Atf 2. Thus, multiple copies of the Tg construct have inserted into Atf2. Sequence analysis demonstrated that truncated versions of ATF-2 transcripts encoding only dimerization and DNA binding domains were expressed in Tg NK cells.

(J:61178, J:127589)
Phenotypes
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Atf2 Mutation:  89 strains or lines available
References
Original:  J:61178 Kim S, et al., In vivo natural killer cell activities revealed by natural killer cell-deficient mice. Proc Natl Acad Sci U S A. 2000 Mar 14;97(6):2731-6
All:  11 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory