Mcm4^{Gt(RRE056)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Mcm4^{Gt(RRE056)Byg}
• Name: minichromosome maintenance complex component 4; gene trap RRE056, BayGenomics
• MGI ID: MGI:3696796
• Synonyms: Mcm4^-
• Gene: Mcm4 Location: Chr16:15441761-15455264 bp, - strand Genetic Position: Chr16, 10.09 cM, cytoband B1
• Alliance: Mcm4^{Gt(RRE056)Byg} page

Mutation origin

• Mutant Cell Line: RRE056
• Germline Transmission: Earliest citation of germline transmission: J:117494
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT2Lxf Vector Type: gene trap
• Mutation details: BayGenomics ES cell line RRE056 contains a gene trap vector inserted right after exon 12. The vector contains the En2 (engrailed 2) intron and subsequent splice acceptor (SA), and beta-geo (B-geo) reporter along with the SV40 polyadenylation signal. The insertion site allows efficient splicing of transcripts from exon 12 to the splice acceptor in the gene trap vector, disrupting the conserved MCM box spanning exons 10-14 of the Mcm4 gene. (J:117494)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRE056	CC200422 ; (MGI Seq Detail)	5' RACE	Chr16:15446458-15447532 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  25 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mcm4 Mutation: 43 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:117494 Shima N, et al., A viable allele of Mcm4 causes chromosome instability and mammary adenocarcinomas in mice. Nat Genet. 2007 Jan;39(1):93-8
• All: 5 reference(s)