Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe
Transgene Detail

Summary

• Symbol: Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe
• Name: transgene insertion 1, Corrinne Lobe
• MGI ID: MGI:3696852
• Synonyms: Grg1 conditional transgene, Grg1^lacZ, iZ/AP-Grg1, Tg(ACTB-Bgeo,-Tle1,-ALPP)1Lbe, Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe
• Transgene: Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe Location: unknown
• Alliance: Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe page

Transgene origin

• Germline Transmission: Earliest citation of germline transmission: J:106684
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Transgene description

• Transgene Type: Transgenic (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  ALPP (250)

  Alpi	(MGI:1924018)

• Mutation details: The transgene contains a strong cytomegalovirus (CMV) enhancer followed by the chicken beta-actin promoter; a loxP-flanked beta-galactosidase/neomycin resistance fusion (Bgeo) gene with three polyadenylation signals; the coding sequence of the mouse transducin-like enhancer of split 1, homolog of Drosophila E(spl) (Tle1) gene; and the human alkaline phosphatase, placental (Regan isozyme) (ALPP) gene preceded by an interal ribosome entry site (IRES). In mice doubly transgenic for this and a selected Cre recombinase (cre) transgene, excision of the floxed Bgeo results in whole-animal or tissue-specific co-expression of Tle1 and the ALPP reporter gene. (J:106684)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:106684 Allen T, et al., Grg1 acts as a lung-specific oncogene in a transgenic mouse model. Cancer Res. 2006 Feb 1;66(3):1294-301
• All: 2 reference(s)