Tg(LCAT)T59Ssf
Transgene Detail

Summary

• Symbol: Tg(LCAT)T59Ssf
• Name: transgene insertion T59, Silvia Santamarina-Fojo
• MGI ID: MGI:3696856
• Synonyms: hLCAT-tg subline T59 (Z line hemizygous 15 copy), line T1
• Transgene: Tg(LCAT)T59Ssf Location: unknown
• Alliance: Tg(LCAT)T59Ssf page

Transgene origin

• Strain of Origin: C57BL/6NCrl

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(LCAT)T59Ssf expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  LCAT (3931)

  Lcat	(MGI:96755)

• Mutation details: The transgene is a 6.2 kb human genomic DNA fragment containing the entire lecithin-cholesterol acyltransferase (LCAT) gene plus 851 bp of 5' and 1,134 bp of 3' flanking sequence. Based on Southern blot analysis, hemizygous mice of this founder line harbor 15 transgene copies. The plasma level of LCAT in mice with this transgene is 11-fold that of endogenous LCAT in non-transgenic mice. Northern blot analysis of various tissues from transgenic mice demonstrates expression of LCAT primarily in the liver, with much weaker expression in testis, brain, heart and lung. (J:118356)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:118356 Vaisman BL, et al., Overexpression of human lecithin cholesterol acyltransferase leads to hyperalphalipoproteinemia in transgenic mice. J Biol Chem. 1995 May 19;270(20):12269-75
• All: 3 reference(s)