Tg(CAG-Bgeo,-DsRed*MST)1Nagy
Transgene Detail

Summary

• Symbol: Tg(CAG-Bgeo,-DsRed*MST)1Nagy
• Name: transgene insertion 1, Andras Nagy
• MGI ID: MGI:3697095
• Synonyms: 11/C1, Tg(ACTB-Bgeo,-DsRed*MST)1Nagy, Tg(ACTB-Bgeo,-DsRed.MST)1Nagy, Tg(CAG-Bgeo,-DsRed*MST)1Nagy, Z/RED
• Transgene: Tg(CAG-Bgeo,-DsRed*MST)1Nagy Location: unknown
• Alliance: Tg(CAG-Bgeo,-DsRed*MST)1Nagy page

Transgene origin

• Germline Transmission: Earliest citation of germline transmission: J:109837
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: The transgene contains the coding sequence for the DsRed*MST variant red fluorescent protein and a polyadenylation signal downstream of a loxP-flanked lacZ/neomycin resistance fusion gene (Betageo) followed by three polyadenylation signals (STOP sequence); both coding sequences reside downstream of a chicken beta-actin promoter and a cytomegalovirus enhancer. Excision of the lacZ-STOP by Cre recombinase in doubly transgenic ES cells or mice leads to expression of DsRed*MST; use of a ubiquitously expressed cre transgene results in fluorescence of ES cells, embryos and adult mice. (J:109837)

Tumor Data

List all tumor models in MMHCdb carrying Tg(CAG-Bgeo,-DsRed*MST)1Nagy

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

The transgenic ES cell clone 11/C1 was used to generate transgenic mice.

The authors originally identified the red fluorescent protein variant employed in constructing the transgene vector as DsRed.T3; in a later erratum, they corrected this to DsRed-MST, which differs from the former at two amino acid positions (K Vintersten et al. 2005. Genesis 42(3):218).

see MMRRC Nagy ES cell lines: ES cell line G4 with DsRed.MST transgene (http://www.mmrrc.org/strains/11988/011988.html) and ES cell line G4 with Z/red transgene (http://www.mmrrc.org/strains/11987/011987.html) - dlb

References

• Original: J:109837 Vintersten K, et al., Mouse in red: red fluorescent protein expression in mouse ES cells, embryos, and adult animals. Genesis. 2004 Dec;40(4):241-6
• All: 23 reference(s)