Tg(Camk2a-cre)10Kmik
Transgene Detail

Summary

• Symbol: Tg(Camk2a-cre)10Kmik
• Name: transgene insertion 10, Katsuhiko Mikoshiba
• MGI ID: MGI:3697157
• Synonyms: TgN(a-CaMKII-nlCre)/10
• Transgene: Tg(Camk2a-cre)10Kmik Location: unknown
• Alliance: Tg(Camk2a-cre)10Kmik page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Camk2a-cre)10Kmik expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Camk2a (MGI:88256)

• Mutation details: Expression of bacteriophage P1 Cre recombinase, bearing the nuclear localization signal peptide of SV40 large T antigen, is directed by the promoter of the mouse gene encoding calcium/calmodulin-dependent protein kinase II alpha. Expression of the transgene was demonstrated by RT-PCR analysis, immunoblot analysis and a reporter assay for Cre recombinase activity. Activity is present specifically in the nuclear fraction of the cerebrum, although a low level (1-10% of that in the cerebrum) of "leaky" expression is observed in the cerebellum as well. (J:104881)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Camk2a (mouse) Summary of all recombinase alleles driven by Camk2a. Alliance mouse Camk2a gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Of several transgenic lines created, lines 10 and 26 exhibit higher Cre recombinase expression than lines 17N, 11 and 20.

References

• Original: J:104881 The RIKEN BioResource Center, Information obtained from The RIKEN BioResource Center. Unpublished. 2006-2013
• All: 5 reference(s)