Apoc1^tm2Lmh
Targeted Allele Detail

Summary

• Symbol: Apoc1^tm2Lmh
• Name: apolipoprotein C-I; targeted mutation 2, Louis M Havekes
• MGI ID: MGI:3697323
• Gene: Apoc1 Location: Chr7:19423405-19426584 bp, - strand Genetic Position: Chr7, 9.94 cM
• Alliance: Apoc1^tm2Lmh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:28261
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A replacement vector was used to disrupt exons 1 and 2 of the Apoc1 gene by replacing them with a 2 kb hygromycin B-resistance gene. The vector was transfected into a heterozygous Apoe-deficient E14 ES cell line. Mice produced from the doubly targeted ES cells disrupted alleles of both Apoe and Apoc1. (J:22858, J:28261)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Apoc1 Mutation: 11 strains or lines available

Notes

Apoc1 disruption in heterozygous Apoe-deficient ES cells was produced using the same construct used to produce Apoc1-deficiency in E14 ES cells. (J:22858)

References

• Original: J:28261 van Ree JH, et al., Inactivation of Apoe and Apoc1 by two consecutive rounds of gene targeting: effects on mRNA expression levels of gene cluster members. Hum Mol Genet. 1995 Aug;4(8):1403-9
• All: 3 reference(s)