Tsix^tm3Jtl
Targeted Allele Detail

Summary

• Symbol: Tsix^tm3Jtl
• Name: X (inactive)-specific transcript, opposite strand; targeted mutation 3, Jeannie T Lee
• MGI ID: MGI:3698272
• Synonyms: deltaDXPas34
• Gene: Tsix Location: ChrX:102475123-102528563 bp, + strand Genetic Position: ChrX, 46.13 cM
• Alliance: Tsix^tm3Jtl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:117335
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129 X (M. m. castaneus x 129)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed neo cassette replaced DXPas34. The neo cassette was subsequently removed via transient cre expression. Absence of a 129-specific BamHI restriction pattern indicated that the 129-derived X chromosome was targeted. (J:117335)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tsix Mutation: 57 strains or lines available

Notes

ES cell line = 16.7, a stable 40XX subclone of ES cell line EL16. 16.7 cells carry one X chromosome from 129 and the other from M. m. castaneus.

References

• Original: J:117335 Cohen DE, et al., The DXPas34 repeat regulates random and imprinted X inactivation. Dev Cell. 2007 Jan;12(1):57-71
• All: 1 reference(s)