Gt(ROSA)26Sor^{tm2(HIF1A/luc)Kael}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2(HIF1A/luc)Kael}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, William G Kaelin
• MGI ID: MGI:3699420
• Synonyms: Gt(ROSA)26Sor^{tm1(HIF1A/luc)Kael}, ODD-Luc, ROSA26 ODD-Luc
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2(HIF1A/luc)Kael} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:118308
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: The human hypoxia-inducible factor 1 alpha sequence coding for amino acids 530-652, including the C-terminal portion of the oxygen-dependent degradation domain (ODD) was fused to the firefly luciferase (luc). The fusion protein was preceded by a loxP-flanked PGK-neo cassette and followed by a bovine polyadenylation sequence. The loxP-flanked neomycin was removed by crossing to a cre-expressing mouse line. In the resulting mice, hypoxia results in stabilization of the fusion protein and enhanced fluorescence detectable in hypoxic tissue. (J:118308)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:118308 Safran M, et al., Mouse model for noninvasive imaging of HIF prolyl hydroxylase activity: assessment of an oral agent that stimulates erythropoietin production. Proc Natl Acad Sci U S A. 2006 Jan 3;103(1):105-10
• All: 29 reference(s)