Mgat4b^tm1Jxm
Targeted Allele Detail

Summary

• Symbol: Mgat4b^tm1Jxm
• Name: mannoside acetylglucosaminyltransferase 4, isoenzyme B; targeted mutation 1, Jamey Marth
• MGI ID: MGI:3699595
• Synonyms: Mgat4bF, Mgat4b type II
• Gene: Mgat4b Location: Chr11:50116162-50125930 bp, + strand Genetic Position: Chr11, 30.37 cM
• Alliance: Mgat4b^tm1Jxm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:212313
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector was used to place a floxed neomycin-thymidine kinase expression cassette downstream of exons 2, 3, and 4, and a loxP site upstream of the exons. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transfected with Cre expression plasmid to excise the neo fragment and leave the floxed exons. (J:101977, J:212313)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mgat4b Mutation: 32 strains or lines available

References

• Original: J:212313 Takamatsu S, et al., Physiological and glycomic characterization of N-acetylglucosaminyltransferase-IVa and -IVb double deficient mice. Glycobiology. 2010 Jan;20(4):485-97
• All: 2 reference(s)