Pclo^tm1Sud
Targeted Allele Detail

Summary

• Symbol: Pclo^tm1Sud
• Name: piccolo (presynaptic cytomatrix protein); targeted mutation 1, Thomas C Sudhof
• MGI ID: MGI:3699630
• Synonyms: Piccolo KO
• Gene: Pclo Location: Chr5:14564932-14913473 bp, + strand Genetic Position: Chr5, 6.27 cM, cytoband A2
• Alliance: Pclo^tm1Sud page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:118451
• Parent Cell Line: SM1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A targeting vector was used to flank exon 14 of the gene with loxP sites and place a neomycin cassette flanked by Frt sites in intron 14 of the gene. Mice developed from chimeras carrying the construct were crossed with a Cre deleter strain effectively removed exon 14 from the genome. (J:118451)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Pclo^tm1Sud/Pclo^tm1Sud	involves: 129S6/SvEvTac

Phenotypes:

Affected Systems	hm1
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normal phenotype	√
no abnormal phenotype detected	√

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pclo Mutation: 267 strains or lines available

References

• Original: J:118451 Sudhof TC, Generation of a Pclo knockout mouse. MGI Direct Data Submission. 2007
• All: 2 reference(s)