St3gal2^tm2Jxm
Targeted Allele Detail

Summary

• Symbol: St3gal2^tm2Jxm
• Name: ST3 beta-galactoside alpha-2,3-sialyltransferase 2; targeted mutation 2, Jamey Marth
• MGI ID: MGI:3699715
• Synonyms: ST3Gal-II^F
• Gene: St3gal2 Location: Chr8:111646554-111699112 bp, + strand Genetic Position: Chr8, 57.81 cM
• Alliance: St3gal2^tm2Jxm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:80119
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector was used to place a floxed neomycin-thymidine kinase expression cassette downstream of the exon containing the large sialyl motif and a loxP site upstream of the exon. Correctly targeted ES cells were transfected with a Cre expression plasmid to excise the neo fragment, leaving the floxed exon. (J:80119)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any St3gal2 Mutation: 37 strains or lines available

References

• Original: J:80119 Ellies LG, et al., Sialyltransferase specificity in selectin ligand formation. Blood. 2002 Nov 15;100(10):3618-25
• All: 2 reference(s)