Tg(KRT14-Esr1/HRAS)1Pkha
Transgene Detail

Summary

• Symbol: Tg(KRT14-Esr1/HRAS)1Pkha
• Name: transgene insertion 1, Paul A Khavari
• MGI ID: MGI:3700054
• Synonyms: K14-ER:Ras
• Transgene: Tg(KRT14-Esr1/HRAS)1Pkha Location: unknown
• Alliance: Tg(KRT14-Esr1/HRAS)1Pkha page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Constitutively active, Humanized sequence, Inducible, Inserted expressed sequence)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(KRT14-Esr1/HRAS)1Pkha expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  HRAS (3265)

  Hras	(MGI:96224)

• Mutation details: The transgene contains a 2075-bp human keratin 14 promoter construct driving expression of a cDNA that encodes a fusion protein comprising a constitutively active mutant form of the catalytic domain of human HRAS, in which glycine at amino acid position 12 is replaced by arginine (G12V), joined at its amino-terminal to a mutant mouse estrogen receptor ligand-binding domain in which replacement of glycine by arginine at amino acid position 525 (G525R, "ER^TM") renders the fusion protein's activity inducible by 4-hydroxytamoxifen, but unresponsive to natural estrogen (17beta-estradiol). (J:55801, J:81660, J:118583, J:118586, J:118587)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Multiple mouse lines were generated, with varying transgene copy numbers and expression levels of the fusion protein. Lines were identified only as H, M, and L for high, medium and low copy number, respectively.

References

• Original: J:81660 Tarutani M, et al., Inducible activation of ras and raf in adult epidermis. Cancer Res. 2003 Jan 15;63(2):319-23
• All: 10 reference(s)