Tg(KRT14-RAF1/ESR1)1Pkha
Transgene Detail

Summary

• Symbol: Tg(KRT14-RAF1/ESR1)1Pkha
• Name: transgene insertion 1, Paul A Khavari
• MGI ID: MGI:3700057
• Synonyms: K14-Raf:ER
• Transgene: Tg(KRT14-RAF1/ESR1)1Pkha Location: unknown
• Alliance: Tg(KRT14-RAF1/ESR1)1Pkha page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Constitutively active, Humanized sequence, Inducible, Inserted expressed sequence)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(KRT14-RAF1/ESR1)1Pkha expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  RAF1 (5894)

  Raf1	(MGI:97847)

• Mutation details: The transgene contains a 2075-bp human keratin 14 promoter construct driving expression of a cDNA that encodes a fusion protein comprising a constitutively active mutant form of the catalytic domain of human RAF1, in which tyrosine is replaced by aspartic acid at amino acid positions 340 and 341 (Y340D/Y341D), joined at its carboxyl-terminal to the ligand-binding domain of human estrogen receptor 1. The fusion protein can be activated in the epidermis by topical application of 4-hydroxytamoxifen. (J:81660, J:118584, J:118585)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Three independent transgenic mouse lines were generated that exhibited similar levels of fusion protein expression.

References

• Original: J:81660 Tarutani M, et al., Inducible activation of ras and raf in adult epidermis. Cancer Res. 2003 Jan 15;63(2):319-23
• All: 5 reference(s)