Rdh12^tm1.1Cahb
Targeted Allele Detail

Summary

• Symbol: Rdh12^tm1.1Cahb
• Name: retinol dehydrogenase 12; targeted mutation 1.1, Christian A Hubner
• MGI ID: MGI:3700208
• Gene: Rdh12 Location: Chr12:79255687-79269438 bp, + strand Genetic Position: Chr12, 35.51 cM, cytoband D2
• Alliance: Rdh12^tm1.1Cahb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:118293
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A floxed neo was cassette inserted into intro 3 and an additional loxP site was placed in the 5' region of the gene via homologous recombination. All of the floxed sequence was then removed by transient Cre expression in the ES cells. Western blot analysis confirmed absence of protein expression in whole eye lysates from homozygous mice. (J:118293)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rdh12 Mutation: 17 strains or lines available

References

• Original: J:118293 Kurth I, et al., Targeted disruption of the murine retinal dehydrogenase gene Rdh12 does not limit visual cycle function. Mol Cell Biol. 2007 Feb;27(4):1370-9
• All: 3 reference(s)