Tg(KRT14-MAP2K1/Esr1)12Pkha
Transgene Detail

Summary

• Symbol: Tg(KRT14-MAP2K1/Esr1)12Pkha
• Name: transgene insertion 12, Paul A Khavari
• MGI ID: MGI:3700387
• Synonyms: CA-Mek1:ER, CA-Mek1:ER (line 12), K14-Mek1:ER (line 12), M1E-12, Mek1:ER
• Transgene: Tg(KRT14-MAP2K1/Esr1)12Pkha Location: unknown
• Alliance: Tg(KRT14-MAP2K1/Esr1)12Pkha page

Transgene origin

• Strain of Origin: FVB

Transgene description

• Transgene Type: Transgenic (Constitutively active, Humanized sequence, Inducible, Inserted expressed sequence)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(KRT14-MAP2K1/Esr1)12Pkha expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  MAP2K1 (5604)

  Map2k1

  (MGI:1346866)

• Mutation details: The transgene contains a 2075-bp human keratin 14 promoter construct with a 5' intron from a beta-globin gene driving expression of a cDNA that encodes a constitutively active mutant human mitogen activated protein kinase 1 (with deletion of amino acids 32-51, which comprise a putative alpha-helical domain, and the amino acid substitutions Ser218Glu and Ser222Asp, which remove phosphorylatively regulable serines). This is joined at its COOH-terminal with the Gly525Arg mutant ligand-binding domain of the mouse estrogen receptor (ER^TM), which renders the fusion protein's activity inducible by 4-hydroxytamoxifen, but unresponsive to natural estrogen (17beta-estradiol). (J:92407, J:118584, J:118587, J:118713, J:118714)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Of three PCR-confirmed transgenic mouse lines, two - designated lines 12 and 13 - express the fusion protein in skin. Topical tamoxifen treatment induces similar skin phenotypes in both lines that are more severe in line 12, which was therefore used in further studies.

References

• Original: J:92407 Scholl FA, et al., Mek1 alters epidermal growth and differentiation. Cancer Res. 2004 Sep 1;64(17):6035-40
• All: 5 reference(s)