Runx1^{tm1(RUNX1/EVI1)Kmit}
Targeted Allele Detail

Summary

• Symbol: Runx1^{tm1(RUNX1/EVI1)Kmit}
• Name: runt related transcription factor 1; targeted mutation 1, Kinuko Mitani
• MGI ID: MGI:3701004
• Synonyms: AML1/EVI1
• Gene: Runx1 Location: Chr16:92398354-92622962 bp, - strand Genetic Position: Chr16, 53.7 cM
• Alliance: Runx1^{tm1(RUNX1/EVI1)Kmit} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:118892
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to replace exon 5 with a chimeric cDNA encoding the human runt related transcription factor 1 exon 5 and the human ecotopic viral integration site 1 into exon 5. Successful expression of the chimeric mice/human transcript was confirmed by Northern blot. (J:118892)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Runx1 Mutation: 34 strains or lines available

References

• Original: J:118892 Maki K, et al., Dysplastic definitive hematopoiesis in AML1/EVI1 knock-in embryos. Blood. 2005 Sep 15;106(6):2147-55
• All: 1 reference(s)