Ighm^tm2.1Bgmn
Targeted Allele Detail

Summary

• Symbol: Ighm^tm2.1Bgmn
• Name: immunoglobulin heavy constant mu; targeted mutation 2.1, Marianne Bruggemann
• MGI ID: MGI:3701077
• Synonyms: Cdelta
• Gene: Ighm Location: Chr12:113382446-113386350 bp, - strand Genetic Position: Chr12, 62.1 cM, cytoband F1-2
• Alliance: Ighm^tm2.1Bgmn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:118453
• Parent Cell Line: ZX3 (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A puromycin resistance gene cassette with a 5' loxP site was inserted into exon 1 and 2 of the Igh Cmu gene segment, deleting 330bp of 3' of exon 1, intron 1 and 5' of exon 2. This insertion was done in ES cells that already had a neo cassette with a 3' loxP site in the alpha 3' enhancer of Igh Calpha (Rr151). Cre mediated recombination results in about a 200 kb deletion spanning the end of exon 1 to the alpha 3' enhancer. (J:118453)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ighm Mutation: 56 strains or lines available

References

• Original: J:118453 Ren L, et al., Silencing of the immunoglobulin heavy chain locus by removal of all eight constant-region genes in a 200-kb region. Genomics. 2004 Oct;84(4):686-95
• All: 5 reference(s)