Igh^tm1Cys
Targeted Allele Detail

Summary

• Symbol: Igh^tm1Cys
• Name: immunoglobulin heavy chain complex; targeted mutation 1, Jason G Cyster
• MGI ID: MGI:3701508
• Synonyms: VDJ9
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:118931
• Parent Cell Line: JM-1 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: An FRT-flanked neomycin resistance cassette was inserted in reverse orientation just downstream of the DQ52 region in a plasmid containing DQ52 (Igh-D). A heavy chain variable-diversity-joining (VDJ) segment of HyHEL10 sequences were placed downstream of the neomycin cassette, replacing the Igh-J (Jh1-4) locus. A loxP site was added to a downstream SpeI restriction site, between the Igh-6 (IgM/mu) enhancer and the Igh-6 switch region, for use in future gene targeting. The immunoglobulin heavy chain locus is of the Igh^a allotype. (J:118931)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 44 strains or lines available

References

• Original: J:118931 Allen CD, et al., Imaging of germinal center selection events during affinity maturation. Science. 2007 Jan 26;315(5811):528-31
• All: 7 reference(s)