Rev1^tm1Ndew
Targeted Allele Detail

Summary

• Symbol: Rev1^tm1Ndew
• Name: REV1, DNA directed polymerase; targeted mutation 1, Niels de Wind
• MGI ID: MGI:3701945
• Gene: Rev1 Location: Chr1:38091867-38168882 bp, - strand Genetic Position: Chr1, 16.46 cM, cytoband B
• Alliance: Rev1^tm1Ndew page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:119176
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 10 was replaced with a PGK-hygro cassette to create a null allele. A western blot using alpha-Rev1 antiserum confirmed the absence of protein in MEF lysates. An RT-PCR of kidney cDNA established the absence of exon 10 in expressed transcripts. (J:119176)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rev1 Mutation: 70 strains or lines available

References

• Original: J:119176 Jansen JG, et al., Strand-biased defect in C/G transversions in hypermutating immunoglobulin genes in Rev1-deficient mice. J Exp Med. 2006 Feb 20;203(2):319-23
• All: 8 reference(s)