Gsk3b^tm1Grc
Targeted Allele Detail

Summary

• Symbol: Gsk3b^tm1Grc
• Name: glycogen synthase kinase 3 beta; targeted mutation 1, Gerald R Crabtree
• MGI ID: MGI:3701999
• Synonyms: GSK-3beta^FRB*
• Gene: Gsk3b Location: Chr16:37909363-38066446 bp, + strand Genetic Position: Chr16, 26.54 cM, cytoband B4
• Alliance: Gsk3b^tm1Grc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:100153
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A mutant FRB tag, composed of an 89 amino acids with three point mutations (K2095P, T2098L, and W2101F), was targeted into the C terminus of the locus. A floxed neo cassette was inserted into the last intron and an FRB*HA sequence placed immediately before the stop codon. The neo was subsequently removed via crossing with mice expressing cre recombinase in the germline. Presence of this mutant FRB tag destabilizes the protein resulting in decrease or loss of expression. Addition of rapamycin or a rapamycin analogue stabilizes the protein and restores expression. (J:100153)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gsk3b Mutation: 113 strains or lines available

References

• Original: J:100153 Stankunas K, et al., Conditional protein alleles using knockin mice and a chemical inducer of dimerization. Mol Cell. 2003 Dec;12(6):1615-24
• All: 5 reference(s)